http://dx.doi.org/10.18081/jcc/016-06/1-8
Journal of Cellular Cancer  Volume 8, Issue 1, pages 1-8
Received December 23, 2015; accepted May 12, 2016; published June 11, 2016

Estabraq AR. Al-Wasiti*

Author Affiliations
*Corresponding Author: Electronic address: estabraqalwasiti@yahoo.com. Dep. of chemistry and biochemistry, Medical College, Al-Nahrain University

Abstract

Male breast cancer is a rare compared to its female counterpart and makes less than 1% of all breast cancer. Risk factors for breast cancer in men, including genetic, hormonal and environmental factors, provide parallels to the etiology of breast cancer in women. To evaluate whether deficient DNA repair contributes to elevated DNA damage and breast carcinogenesis, this study used comet assay (single-cell alkaline gel electrophoresis) to measure the levels of DNA damage in peripheral leukocytes and tissue from 20 breast tumor cases and 20 controls (only peripheral leukocytes). The results showed that cancer cases had significant higher DNA damage compared with controls and benign cases, the comet tail moments (mean ±SD) for cases (benign and cancer) and controls were: 5.64±0.88, 7.93±0.82 and 3.18±0.74 respectively. For tissue the cancer cases showed higher DNA damage compared with begin breast tumors (15.33±0.92 and 11.24± 1.57). Smoking status was causes to increase DNA damage in the Leukocyte of the patients with benign breast tumors and breast cancer when compared to non smoking cases the (mean ±SD) for benign (5.17±0.71 versus 6.47±0.41) while for malignant tumors were (7.25±0.62 versus 8.0±0.39). As body mass index (BMI) increase will causes increases in DNA damage for benign and malignant breast tumor. In conclusion our results support the hypothesis that increased breast cancer risk is associated with higher DNA damage and evaluation of DNA damage response may contribute to early detection and prevention of breast cancer.

Keywords: Comet assay; Oxidative DNA damage; Male breast cancer

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