Cytotoxicity and modulation of synthesized nitrochalcone derivative on rhabdomyosarcoma cell line

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http://dx.doi.org/10.18081/jcc/016-06/41-51
Journal of Cellular Cancer Volume 8, Issue 1, pages 41-51
Received March 01, 2016; accepted May 08, 2016; published June 28, 2016

Khalida K. Abbas Al-Kelaby1*, Samer A. Hasan2, Jawad K. Abbas3

Author Affiliations
*Corresponding Author: Electronic address: khalida_kadim@yahoo.com ¹Department of Clinical and Laboratory Sciences, College of Pharmacy. ²Department of Pharmaceutical Chemistry, College of Pharmacy. ³Department of Internal Medicine, College of Medicine.

Abstract

Many efforts have focused on the therapeutic approach of cancer for a long time by studying of cytotoxic activities of plant constituents. This study was planned to evaluate cytotoxicity of nitrochalcone derivative (NCD) on rhabdomyosarcoma cell line (RMS) as compared with methotrexate (MTX) and Doxorubicin (DOX), then assessment of monoterpene (MT1) combination with NCD. The results of current study were showed that NCD was effectively inhibited the cell proliferation by decreasing the viability of RMS cells at different concentrations involved 0.5, 2.5, 10, 20, 30, 40, 50, 60 and 70 μg/ml with IC50 of 2.117μg/ml, while DOX and MTX IC50 values were 0.553μg/ml and 0.8708μg/ml respectively. This study was also revealed combined effect of NCD with MT1 (IC50 = 2.0667). We concluded that NCD have an anticancer effect that can be modulated by monoterpenes.

Keywords: Nitrochalcone derivative; Cytotoxicity assay; RMS cell line

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